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Expansion was determined by enumerating the cell aggregates obtained at harvest and dividing by the volume of cell aggregates seeded. Take note this information is representative of cultures passaged after 6-seven times in culture, lower enlargement should be envisioned if working with shorter lifestyle moments.
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Use printed cryo labels or simply a marker immune to both alcohol and liquid nitrogen. It is also good practice to maintain a list of banked cells and history Any time a vial is additional to or faraway from storage. This could support ensure you sustain Stem cell an satisfactory stock of frozen cells.
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